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metI [2019-01-28 15:46:42]
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metI [2019-01-28 15:46:42]

O-succinylhomoserine lyase (L-cysteine, H2S, methanethiol, elimination)
Locus
BSU11870
Isoelectric point
5.02
Molecular weight
41.55 kDa
Protein length
373 aa Sequence Blast
Gene length
1119 bp Sequence Blast
Function
biosynthesis of methionine
Product
O-succinylhomoserine lyase (L-cysteine, H2S, methanethiol, elimination)
Essential
no
Synonyms
yjcI
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Amino acid/ nitrogen metabolism → Biosynthesis/ acquisition of amino acids → Biosynthesis/ acquisition of methionine/ S-adenosylmethionine

    • Gene

    Coordinates
    1,258,492 → 1,259,613

    The protein

    Paralogous protein(s)

  • MetC, MccB

    • Structure

  • 4L0O (from Helicobacter pylori, 48% identity)

    • Expression and Regulation

    Operons

    Genes
    metI-metC
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulatory mechanism

  • S-box: transcription termination/ antitermination, the S-box riboswitch binds S-adenosylmethionine resulting in termination, in S-box

    • Regulation

  • induced by methionine starvation (S-box) PubMed
  • the S-box RNA is degraded by RNase Y PubMed
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    Biological materials

    Mutant

  • MGNA-B298 (yjcI::erm), available at the NBRP B. subtilis, Japan
  • 1A941 ( metI::spec), PubMed, available at BGSC
  • BKE11870 (ΔmetI::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATAGCTGACACCTTCCC, downstream forward: _UP4_CAGGTCAAAGAGGGAGCTGT
  • BKK11870 (ΔmetI::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATAGCTGACACCTTCCC, downstream forward: _UP4_CAGGTCAAAGAGGGAGCTGT

    • References

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